13^th World Pediatric Congress

Introduction & Aim: Qualitative and quantitative deviations of the intestinal microbiota provoke a number of diseases. The purpose of this study is to determine the significance of Prevotella enterotype with Functional Gastrointestinal Disorders (FGID) in children.

 

Materials & Methods: We examined 76 children at the age 1-18 years, among which 57 patients were diagnosed different FGID in accordance to Rome IV diagnostic criteria. The rest of the children without any digestive system pathology, constituted the reference group (n=21). Prevotella and P. copri identification was carried out in fecal supernatant by real-time PCR using of

specific primers Prevotella and P. copri.

 

Results: The leading FGID were the Irritable Bowel Syndrome (IBS) 54.4% and Functional Disorders of the Biliary Tract (FDBT) 45.6%. Representatives of the Prevotella genus in children with FGID were detected in 35 (61.4%), but in children of the reference group Prevotella was found in 90.5%, which revealed a significant connection (χ2=5.0; p=0.03) between the detection of Prevotella and the presence of FGID. P. copri amount in patients with FGID was 2-fold lower (106.9±8.4), than reference values (250.4±7.3). The mean value of P. copri in the fecal supernatant in children with IBS was (80.7±11.2) Relative Units (RU), and in children with FDBT ((124.3±10.5) RU) (p<0.05). It was shown that absence of P. copri and its minimum level are characterized by a high association with IBS with prevalence of constipation (χ2=6.0; p=0.02).

 

Conclusion: In children with FGID the intestinal microbiota is characterized by the absence of Prevotella in 38.6% (95% CI 51.4-25.8) and decreased count in 45.6% (95% CI 58.8-32.4). It was shown that decreased count of P. copri or its absence is associated with IBS (constipation) (OR 3.9; CI 1.3-12.0), which indicates to more profound intestinal microbiota disturbance in children with functional bowel disorders.